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Why is it so important to make a standard curve
Because of its easy operation,enzyme-linked immunosorbent assay (ELISA) can simply answer the basic question of how many proteins/polypeptides/antibodies are in the sample. It is a commonly used detection method in scientific research.The core of this simple analysis method is the standard curve.Wit
2023-08-02
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How Green Hand operates ELISA experiment?
How Green Hand operates ELISA experiment? That's a good question, will she get a satisfactory test result? and what precautions should be paid attention to? Let's check this video recorded in ELK lab.
2022-07-27
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Mycoplasma simple detection method
Extract the RNA of the cells to be tested by Trizol method (recommended to use TRIpure Total RNA Extraction Reagent Cat. No.: EP013), reverse transcribed into cDNA Detection primer PCR detection of Mycoplasma contamination of cells. Mycoplasma detection primers
2022-06-23
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Proficiently manipulate the UniProt database
1,Introduction and use details of Uniprot protein database Uniprot database is the most widely resourced and most informative protein database, and the first choice for querying protein functions. The Uniprot database consists of three sub-databases, Swiss-Prot, TrEMBL and PIR-PSD. The data mainl
2022-06-09
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A brief introduction to the principle and detailed experimental steps of co-immunoprecipitation (Co-IP)
1.Principle Co-immunoprecipitation (Co-Immunoprecipitation) is a classic method for studying protein interactions based on the specific interaction between antibodies and antigens. It is an effective method to determine the physiological interaction of two proteins in intact cells. &
2022-06-08
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RNA Sample Preparation Guide
1、RNA SAMPLE yield and minimum input amount for various projects Total RNA yield statistics of each sample (for reference only)： Recommended starting amount for sequencing projects（the starting amount is for reference only,exosomes do not allow 18/s28s rRNA peaks）： Expres
2022-06-08
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What is ELISA--Principle and Classification
1. Basic Principles In 1971, Engvall and Perlmann published an article on enzyme-linked immunosorbent assay (ELISA) for the quantitative determination of IgG, which made the enzyme-labeled antibody technology for antigen localization developed in 1966 to be used for the determination of trace
2022-05-23
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How to choose loading control antibodies
The loading control antibody can be used to evaluate the efficiency of Western Blot and compare the amount of protein loaded per well in the gel. Such controls can help identify differences in expression levels between samples, whether due to actual protein levels in cell lysates or d
2022-05-19