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choosing the right ELISA kit can help you achieve more with less effort.
1.What is ELISA Kit？ Enzyme-linked immunosorbent assay ( ELISA ) is the most widely used technology in enzyme immunoassay technology . The invention of ELISA Kit has helped scientific researchers save the tedious experimental process and improved scientific research efficiency. Choosing the
2025-02-20
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Collection, processing and storage of tissue homogenate and cell lysate samples
Tissue homogenate Tissue homogenization refers to an experimental technique in which tissue samples are broken up and evenly dispersed in buffer for subsequent experimental manipulation. In the following we will detail the procedure and precautions for tissue homogenization. 1. T
2024-12-30
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Why is it so important to make a standard curve
Because of its easy operation,enzyme-linked immunosorbent assay (ELISA) can simply answer the basic question of how many proteins/polypeptides/antibodies are in the sample. It is a commonly used detection method in scientific research.The core of this simple analysis method is the standard curve.Wit
2023-08-02
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How Green Hand operates ELISA experiment?
How Green Hand operates ELISA experiment? That's a good question, will she get a satisfactory test result? and what precautions should be paid attention to? Let's check this video recorded in ELK lab.
2022-07-27
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Mycoplasma simple detection method
Extract the RNA of the cells to be tested by Trizol method (recommended to use TRIpure Total RNA Extraction Reagent Cat. No.: EP013), reverse transcribed into cDNA Detection primer PCR detection of Mycoplasma contamination of cells. Mycoplasma detection primers
2022-06-23
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Proficiently manipulate the UniProt database
1,Introduction and use details of Uniprot protein database Uniprot database is the most widely resourced and most informative protein database, and the first choice for querying protein functions. The Uniprot database consists of three sub-databases, Swiss-Prot, TrEMBL and PIR-PSD. The data mainl
2022-06-09
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A brief introduction to the principle and detailed experimental steps of co-immunoprecipitation (Co-IP)
1.Principle Co-immunoprecipitation (Co-Immunoprecipitation) is a classic method for studying protein interactions based on the specific interaction between antibodies and antigens. It is an effective method to determine the physiological interaction of two proteins in intact cells. &
2022-06-08
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RNA Sample Preparation Guide
1、RNA SAMPLE yield and minimum input amount for various projects Total RNA yield statistics of each sample (for reference only)： Recommended starting amount for sequencing projects（the starting amount is for reference only,exosomes do not allow 18/s28s rRNA peaks）： Expres
2022-06-08