-
What is ELISA--Principle and Classification
1. Basic Principles In 1971, Engvall and Perlmann published an article on enzyme-linked immunosorbent assay (ELISA) for the quantitative determination of IgG, which made the enzyme-labeled antibody technology for antigen localization developed in 1966 to be used for the determination of trace
2022-05-23
-
How to choose loading control antibodies
The loading control antibody can be used to evaluate the efficiency of Western Blot and compare the amount of protein loaded per well in the gel. Such controls can help identify differences in expression levels between samples, whether due to actual protein levels in cell lysates or d
2022-05-19
-
ELK Biotechnology Collates Protein Extraction Methods for Westernblot Experiments
Westernblot experiment protein extraction 1) Total protein extraction from adherent cells. Wash the adherent cells with TBS buffer 2-3 times, aspirating the residue as much as possible on the last occasion. Add the appropriate volume of total cell protein extraction reagent (add protease inhib
2022-05-19
-
Calculation of ELISA results by two software
Method 1: Use OriginPro 2021 software to calculate the ELISA results of the competition kit Step 1: We take PG(Progesterone) ELISA Kit as an example Copy and paste the data of the standard curve into the following table. Press Ctrl+A to select all and then click&n
2022-05-11
-
Westernblot experimental protein extraction
Adherent cell total protein extraction: Rinse the adherent cells 2-3 times with TBS buffer, blotting the remaining liquid as much as possible the last time. Add an appropriate volume of cell total protein extraction reagent (add protease inhibitor within minutes before use) and lyse in
2022-05-11
-
Instructions for primer dry powder
(1) Centrifuge dry primer powder at 13,000 rpm for 1 min at room temperature before opening the lid. (2) Add DEPC water with 10 times nmole value of water (μL) on the tube wall, vortex to mix, and centrifuge briefly. This is the primer stock solution (100 μM), which is stored at -
2022-05-11
-
Analysis of common problems and causes of ELISA experiment
High background/non-specific staining Description of results Possible reason Recommendations and Precautions After termination, the whole plate results show a uniform yellow or light color; or the standard curve is linear but
2022-05-04
-
ELISA experiment operation video
2022-04-26