Technical

Mycoplasma simple detection method
source:Technical divisiondate:2022-06-23views:1072

Extract the RNA of the cells to be tested by Trizol method (recommended to use TRIpure Total RNA Extraction Reagent Cat. No.: EP013), reverse transcribed into cDNA

 

Detection primer PCR detection of Mycoplasma contamination of cells.

 

Mycoplasma detection primers

 

 

Mycoplasma_F01:

 

GGG AGC AAA CAG GAT TAG TAT CCC T

 

 

Mycoplasma_R01:

 

TGC ACC ATC TGT CAC TCT GTT AAC CTC

 

 

40 cycles, annealed at 63 degrees

 

Product size: about 200bp

 

Positive: Mycoplasma infection; Negative: Not infected

 

ELK Biotechnology® has been deeply involved in the field of immunology for more than 10 years, and has accumulated a lot of experience in ELISA R&D and production. The existing technology and antibody pair reserves can produce more than 8,000 high-quality Elisa kits, covering more than 50 countries and regions around the world. It has won unanimous praise from the majority of scientific research users.

Excellent product performance

 

High accuracy:The inter-assay difference and intra-assay coefficient of variation are both less than 9.9%

High sensitivity: PG level

High specificity: The cross-reaction rate is less than 5%

Excellent recovery rate: 85%--115%

High stability: After the reagents are stored at different temperatures, the maximum use time is up to 1 year