ELK Biotechnology Kits Facilitate Cell Metabolism Research

Cell Metabolism

Cell metabolism is a series of orderly chemical reactions that sustain life, involving a range of complex biochemical processes. Proper functioning of these reactions is crucial for maintaining health, and metabolic abnormalities can lead to severe consequences such as metabolic diseases, tumors, and neurodegenerative disorders.

Currently, glucose metabolism, lipid metabolism, and oxidative stress are hotspots in the field of cell metabolism research. Research on cell metabolism mainly relies on two types of assays:

1.Metabolite Assays: Quantitative analysis of specific metabolites in cells using specific kits or metabolomics methods. By measuring changes in the levels of different metabolites, researchers can understand the metabolic state of cells and determine whether certain metabolic pathways are activated or inhibited.

2.Metabolic Enzyme Assays: These aim to determine the activity of rate-limiting enzymes in specific metabolic pathways. By measuring enzyme activity levels, researchers can understand the enzyme's activity and the corresponding metabolite content, revealing the regulatory mechanisms of the pathway and changes in key components.

ELK Biotechnology, a globally recognized biotechnology company, offers a variety of high-quality, user-friendly cell metabolism assay kits. Using our kits, researchers can gain in-depth insights into the role and impact of metabolism in various diseases, thereby accelerating the diagnosis and treatment processes.

Product Advantages

Advantages of ELK Biotechnology Metabolism Assay Kits:

• High Compatibility: Suitable for various biological samples and can be tested on common laboratory instruments.
• High Stability: Provides high-quality experimental results with high reproducibility and stability.
• Wide Selection: Offers kits for key metabolic pathways for you to choose from.
• User-Friendly: Simple and easy experimental procedures, suitable even for beginners.

Recommended Kits for Key Metabolites in Metabolic Pathways

Glucose Metabolism

The glycolysis pathway is an essential metabolic process in cells, breaking down glucose into two molecules of pyruvate and generating energy. This pathway includes six steps, with three rate-limiting steps determining the overall pathway rate. Key enzymes include hexokinase, phosphofructokinase-1, and pyruvate kinase. ELK Biotechnology assay kits can easily measure the activity of these enzymes and the corresponding metabolite content.

Lipid Metabolism

Fatty acid metabolism is vital for energy production and storage. When fatty acids are released from fat cells, they circulate in the blood as free fatty acids and then enter cells for breakdown, producing energy. ELK Biotechnology assay kits can be used to study the breakdown and synthesis processes of fatty acids.

Tricarboxylic Acid Cycle

The tricarboxylic acid cycle is a crucial metabolic pathway in organisms, where acetyl-CoA is oxidized to carbon dioxide, releasing energy. ELK Biotechnology assay kits can be used to study the enzyme activity and synthesis processes in this pathway.

Oxidative Stress

Oxidative stress refers to the imbalance between oxidation and antioxidation in the body, leading to increased inflammatory infiltration by neutrophils and secretion of proteases, producing a large number of oxidative intermediates. Oxidative stress is caused by the production of free radicals in the body and is a major factor in aging and disease. ELK Biotechnology assay kits can be used to study enzyme activity and synthesis processes during oxidative stress.

Selected Literature References

1. Arora S, Kumar V, Kapil L, Agrawal AK, Singh A, Singh C. Piperine loaded metal-organic frameworks reverse doxorubicin-induced chemobrain in adult zebrafish. J Control Release. 2023;355:259-272. doi:10.1016/j.jconrel.2023.01.077
2. Shang Q, Yang Y, Li H. LINC01605 knockdown induces apoptosis in human Tenon's capsule fibroblasts by inhibiting autophagy. Exp Ther Med. 2022;23(5):343. doi:10.3892/etm.2022.11273
3. Hu N, Zeng X, Tang F, Xiong S. Exosomal long non-coding RNA LIPCAR derived from oxLDL-treated THP-1 cells regulates the proliferation of human umbilical vein endothelial cells and human vascular smooth muscle cells. Biochem Biophys Res Commun. 2021;575:65-72. doi:10.1016/j.bbrc.2021.08.053
4. Xin Y, Zhang Y, Deng S, Hu X. Vagus Nerve Stimulation Attenuates Acute Skeletal Muscle Injury Induced by Hepatic Ischemia/Reperfusion Injury in Rats. Front Pharmacol. 2022;12:756997. Published 2022 Jan 3. doi:10.3389/fphar.2021.756997