The nude mouse tumor model (Cell Derived Xenograft, CDX) is a commonly used tumor research model, which involves directly injecting tumor cells cultured in vitro into immunodeficient mice (usually nude mice, i.e. BALB/c-nu mice) to simulate the biological characteristics of human tumors, including tumor formation, development, metastasis and other processes. This model is widely used in the screening and efficacy evaluation of anti-tumor drugs, helping researchers understand the inhibitory effect of drugs on tumor growth.

Experimental Materials and Methods

Experimental Materials: BALB/c-nu mice (male, 18-22g); Panc02 cells (5*106/200μL)

Modeling Method

1. Adaptive feeding for 7 days, subcutaneous injection of 200μL of cancer cells into each nude mouse.

2. Observe the growth of subcutaneous tumors in nude mice after 3-5 days, and observe and record the growth of subcutaneous tumors in nude mice every two days after 1 week.

3. Samples were collected around 28 days and HE staining was performed.

Evaluation indicators

Animal gross image and tumor photography, tumor growth curve, HE staining.

HE staining results: Tumor cells are small, round, oval or short spindle-shaped, with scarce cytoplasm and unclear cell boundaries. A small number of tumor cells have nuclear condensation, apoptosis, and necrosis.