1.Is there a reward for publishing papers using ELK ELISA Kit?

If you use our ELISA Kit and have published a paper, you can get a reward of up to 300 USD, please contact our sales staff for the policy.

2.What is the storage temperature of the kit after receipt? Can it be placed at -20°C?

Unopened kit can be stored at 4° C 6 months; -20° C 12 months

3.How about the accuracy and stability of your kits? How long is the shelf life? There won't be any problem during transportation, right?

The accuracy and stability of our kits are very good, all the products have been verified by thermal stability experiments in the R&D stage, and must undergo a three steps quality check before shipment, and the special stabilizers developed by ELK can well guarantee the activity of each reagent. The validity period is 4° C 6 months, -20° C 12 months, to ensure that the reagent kit 4 degrees transportation is safer and more stable

4.What is the purification method of the antibody in the kit?

Affinity chromatography

5.Can your kits be used for clinical diagnosis?

Research use only, not for clinical diagnosis.

6.How many samples can be tested in one kit?

7.How should the standard be stored after dissolution? 4℃? How long can it be stored after dissolution?

The standard should be preparsd right before use, and used as soon as possible within 24 hours after dissolution to avoid inaccurate quantification due to partial degradation. The stock solution can be stored until -20 and then used within one week, other concentrations of liquid can not be stored, the next timeyou use the liquid need to be re-diluted from the stock solution

8.Can you provide a more concentrated standard so that the detection range can cover the concentration of my sample?

The maximum value and detection range of the standards in the kit are determined by the assay data at the R&D stage and cannot be changed arbitrarily. If the sample concentration is too high, it can be diluted. The dilution ratio can be determined by pre-test. Generally speaking, if the sample concentration is too high, the detection can be completed by dilution. If there is a definite need for a kit with the appropriate range and sensitivity, customized services are available.

9.I don't know how many times my sample should be diluted? Can you provide me with a detailed description?

The dilution should be determined by pre-experiment (for the accuracy of the experiment, make a prediction of the target protein content of the sample first. Then compare the detection range of the kit, if it exceeds the corresponding dilution, it is recommended to take 2-3 samples, each sample to do a gradient dilution, such as (stock solution, 1:5, 1:20), the detection of the sample OD in the range of the standard curve S2_54, for the optimal dilution of the sample)

10.Can saline be used for sample dilution?

In order to ensure that all wells are in the same condition, please use the sample diluent provided in the kit, not saline or other self-prepared reagents when diluting the samples.

11.Can this kit be used to test cell lysate? If yes, is it possible to use the lysate directly, or do we need to separate the nucleus part of the cell?

Cell lysate can be detected, and the supernatant can be centrifuged directly after cell lysis.

12.How should the data be calculated after the experiment?

Reply: The data measured in the experiment can be imported into the professional curve software CurveExpert1.38 or 1.4, which can help you automatically match a most suitable curve. Please refer to the technical support article in our website for the specific operation steps.

13.How much sample volume do I need to prepare?

Reply: Generally, 100ul is required, 200ul is required for 1 duplicate well and so on (only 50ul is required for the competition method). If it is determined that the sample needs to be diluted a certain number of times, the sample amount can be reducedaccordingly. For ELISA kits using the sandwich principle, a sample volume of 100ul is required for a single-well test; for ELISA kits using the competition principle, a sample volume of 50ug is required for a single-well test; in addition, we have also launched a version of the micro-volume sampling method, which requires a sample volume of only 25ul for a single-well test, please feel free to contact us.

14.Concentrated biotinylated antibody and HRP enzyme conjugate are not enough?

The reagents in the kit are shipped in sufficient quantities in consideration of packaging and usage losses. so there is no risk of running out of reagents for normal use. Before use, centrifugation is required to minimize the loss of tube wall adhesion, and because multiple experiments will Increase the loss, it is necessary to pay attention to the control of loss when using in batches.

ELISA PRECAUTIONS

1. Please store the kit according to the instructions before use. If the standard is not used up in time after reconstitution, please discard it.

2.Concentrated Biotinylated Antibody and Concentrated Enzyme Conjugate are small in size and may be dispersed in various parts of the centrifuge tube during transportation. Centrifuge the tube at 1000×g for 1 minute before use to allow liquid from the wall or cap to settle to the bottom of the tube. Pipette the solution 4-5 times before use to mix the solution. The standard, biotinylated antibody working solution, and enzyme conjugate working solution should be configured according to the required dosage, and the corresponding dilution solution should be used.

3.The concentrated washing solution taken out from the refrigerator may have crystals, this is a normal phenomenon, you can let the crystals dissolve completely in the water bath or incubator before configuring the washing solution (the heating temperature should not exceed 40℃). The washing solution should be room-mixed at the time of use.

4. Sample addition should be done quickly, preferably within 10 minutes each time, and it is recommended to set up duplicate wells to ensure the accuracy of the experiment. When pipetting reagents, maintain a consistent order of addition from one well to another, which will ensure that all wells have the same incubation time.

5. The residual washing solution in the reaction wells during washing should be patted dry on absorbent paper, do not put the filter paper directly into the reaction wells to absorb water, and pay attention to removing the residual liquid at the bottom and fingerprints before reading so as not to affect the enzyme marker reading.

6. Avoid direct exposure to strong light during storage and use of the color developer TMB. After adding the substrate, pay attention to observe the color change in the reaction wells, the suggested incubation time is 20 minutes, and the color development time can be extended appropriately if the color development is not sufficient, but should not exceed 30 minutes.

7.The ELISA plate materials and reagents used during the experiment are all disposable, and repeated use is strictly prohibited, otherwise the results will be affected.

8. Please wear lab coat and latex gloves for protection during the experiment, especially when testing blood or other body fluid samples, according to the national regulations on safety and protection in biological laboratories.

9. Components of the kit of different lot numbers should not be mixed (except washing solution and reaction termination solution).

10. The enzyme strips in the kit are detachable, please use them in batches according to the experimental needs; the remaining kit is recommended to be used up within 1 month after the first experiment.