PCNA rabbit pAb

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PCNA rabbit pAb

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ES8839

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50 µL	$148.00
100 µL	$248.00

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Overview

Product name:	PCNA rabbit pAb
Reactivity:	Human;Mouse;Rat
Alternative Names:	Proliferating cell nuclear antigen (PCNA) (Cyclin)
Source:	Rabbit
Dilutions:	WB 1:500-2000;IHC-p 1:50-300; ELISA 2000-20000
Immunogen:	Synthesized peptide derived from human PCNA. at AA range: Internal
Storage:	-20°C/1 year
Clonality:	Polyclonal
Isotype:	IgG
Concentration:	1 mg/ml
Observed Band:	30-33kd
GeneID:	5111
Human Swiss-Prot No:	P12004
Cellular localization:	Nucleus . Colocalizes with CREBBP, EP300 and POLD1 to sites of DNA damage (PubMed:24939902). Forms nuclear foci representing sites of ongoing DNA replication and vary in morphology and number during S phase (PubMed:15543136). Co-localizes with SMARCA5/SNF2H and BAZ1B/WSTF at replication foci during S phase (PubMed:15543136). Together with APEX2, is redistributed in discrete nuclear foci in presence of oxidative DNA damaging agents. .
Background:	The protein encoded by this gene is found in the nucleus and is a cofactor of DNA polymerase delta. The encoded protein acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage, this protein is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway. Two transcript variants encoding the same protein have been found for this gene. Pseudogenes of this gene have been described on chromosome 4 and on the X chromosome. [provided by RefSeq, Jul 2008],

Western Blot analysis of Hela cells using primary antibody diluted at 1:1000(4°C overnight). Secondary antibody:Goat Anti-rabbit IgG IRDye 800( diluted at 1:5000, 25°C, 1 hour) cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit
Immunohistochemical analysis of paraffin-embedded human liver cancer. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).