COX4I2 rabbit pAb

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COX4I2 rabbit pAb

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ES7777

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50 µL	$148.00
100 µL	$248.00

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Overview

Product name:	COX4I2 rabbit pAb
Reactivity:	Human;Mouse;Rat
Alternative Names:	COX4I2; COX4L2; Cytochrome c oxidase subunit 4 isoform 2; mitochondrial; Cytochrome c oxidase subunit IV isoform 2; COX IV-2
Source:	Rabbit
Dilutions:	Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications.
Immunogen:	The antiserum was produced against synthesized peptide derived from human COX42. AA range:31-80
Storage:	-20°C/1 year
Clonality:	Polyclonal
Isotype:	IgG
Concentration:	1 mg/ml
Observed Band:	20kD
GeneID:	84701
Human Swiss-Prot No:	Q96KJ9
Cellular localization:	Mitochondrion inner membrane ; Single-pass membrane protein .
Background:	Cytochrome c oxidase (COX), the terminal enzyme of the mitochondrial respiratory chain, catalyzes the electron transfer from reduced cytochrome c to oxygen. It is a heteromeric complex consisting of 3 catalytic subunits encoded by mitochondrial genes and multiple structural subunits encoded by nuclear genes. The mitochondrially-encoded subunits function in electron transfer, and the nuclear-encoded subunits may be involved in the regulation and assembly of the complex. This nuclear gene encodes isoform 2 of subunit IV. Isoform 1 of subunit IV is encoded by a different gene, however, the two genes show a similar structural organization. Subunit IV is the largest nuclear encoded subunit which plays a pivotal role in COX regulation. [provided by RefSeq, Jul 2008],

Western Blot analysis of K562 insulin 0.01U/ml 15' cells using COX4I2 Polyclonal Antibody
Immunofluorescence analysis of COS7 cells, using COX42 Antibody. The picture on the right is blocked with the synthesized peptide.
Western blot analysis of lysates from K562 cells, treated with insulin 0.01U/ml 15', using COX42 Antibody. The lane on the right is blocked with the synthesized peptide.
Immunohistochemical analysis of paraffin-embedded human Squamous cell carcinoma of lung. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).