Wee1 (phospho Ser642) rabbit pAb

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Wee1 (phospho Ser642) rabbit pAb

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ES7515

Size：	Price：
50 µL	$148.00
100 µL	$248.00

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Overview

Product name:	Wee1 (phospho Ser642) rabbit pAb
Reactivity:	Human;Mouse;Rat
Alternative Names:	WEE1; Wee1-like protein kinase; WEE1hu; Wee1A kinase
Source:	Rabbit
Dilutions:	Western Blot: 1/500 - 1/2000. ELISA: 1/5000. Not yet tested in other applications.
Immunogen:	The antiserum was produced against synthesized peptide derived from human WEE1 around the phosphorylation site of Ser642. AA range:597-646
Storage:	-20°C/1 year
Clonality:	Polyclonal
Isotype:	IgG
Concentration:	1 mg/ml
Observed Band:	100kD
GeneID:	7465
Human Swiss-Prot No:	P30291
Cellular localization:	Nucleus.
Background:	WEE1 G2 checkpoint kinase(WEE1) Homo sapiens This gene encodes a nuclear protein, which is a tyrosine kinase belonging to the Ser/Thr family of protein kinases. This protein catalyzes the inhibitory tyrosine phosphorylation of CDC2/cyclin B kinase, and appears to coordinate the transition between DNA replication and mitosis by protecting the nucleus from cytoplasmically activated CDC2 kinase. [provided by RefSeq, Jul 2008],

Western Blot analysis of 293 cells using Phospho-Wee1 (S642) Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA).
Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using WEE1 (Phospho-Ser642) Antibody
Western blot analysis of lysates from 293 cells treated with etoposide 25uM 60', using WEE1 (Phospho-Ser642) Antibody. The lane on the right is blocked with the phospho peptide.