Thymidine Kinase rabbit pAb

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Thymidine Kinase rabbit pAb

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ES7397

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50 µL	$148.00
100 µL	$248.00

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Overview

Product name:	Thymidine Kinase rabbit pAb
Reactivity:	Human;Monkey
Alternative Names:	TK1; Thymidine kinase; cytosolic
Source:	Rabbit
Dilutions:	WB 1:500-2000;IHC-p 1:50-300
Immunogen:	The antiserum was produced against synthesized peptide derived from human KITH. AA range:161-210
Storage:	-20°C/1 year
Clonality:	Polyclonal
Isotype:	IgG
Concentration:	1 mg/ml
Observed Band:	25kD
GeneID:	7083
Human Swiss-Prot No:	P04183
Cellular localization:	Cytoplasm.
Background:	catalytic activity:ATP + thymidine = ADP + thymidine 5'-phosphate.,miscellaneous:Two forms have been identified in animal cells, one in cytosol and one in mitochondria. Activity of the cytosolic enzyme is high in proliferating cells and peaks during the S-phase of the cell cycle; it is very low in resting cells.,PTM:Phosphorylated on Ser-13 in mitosis.,similarity:Belongs to the thymidine kinase family.,

Western blot analysis of lysates from HeLa and COS7 cells, using KITH Antibody. The lane on the right is blocked with the synthesized peptide.
Western blot analysis of the lysates from RAW264.7cells using KITH antibody.
Immunohistochemical analysis of paraffin-embedded human liver cancer. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).