PCB rabbit pAb

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PCB rabbit pAb

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ES6488

Size：	Price：
50 µL	$148.00
100 µL	$248.00

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Overview

Product name:	PCB rabbit pAb
Reactivity:	Human;Mouse;Rat
Alternative Names:	PC; Pyruvate carboxylase; mitochondrial; Pyruvic carboxylase; PCB
Source:	Rabbit
Dilutions:	Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. IF 1:100-300 Not yet tested in other applications.
Immunogen:	The antiserum was produced against synthesized peptide derived from human PC. AA range:357-406
Storage:	-20°C/1 year
Clonality:	Polyclonal
Isotype:	IgG
Concentration:	1 mg/ml
Observed Band:	120kD
GeneID:	5091
Human Swiss-Prot No:	P11498
Cellular localization:	Mitochondrion matrix .
Background:	This gene encodes pyruvate carboxylase, which requires biotin and ATP to catalyse the carboxylation of pyruvate to oxaloacetate. The active enzyme is a homotetramer arranged in a tetrahedron which is located exclusively in the mitochondrial matrix. Pyruvate carboxylase is involved in gluconeogenesis, lipogenesis, insulin secretion and synthesis of the neurotransmitter glutamate. Mutations in this gene have been associated with pyruvate carboxylase deficiency. Alternatively spliced transcript variants with different 5' UTRs, but encoding the same protein, have been found for this gene. [provided by RefSeq, Jul 2008],

Immunofluorescence analysis of A549. 1,primary Antibody was diluted at 1:200(4°C overnight). 2, Goat Anti Rabbit IgG (H&L) - Alexa Fluor 488 Secondary antibody was diluted at 1:1000(room temperature, 50min).3, Picture B: DAPI(blue) 10min.
Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue, using PC Antibody. The picture on the right is blocked with the synthesized peptide.
Western blot analysis of lysates from Jurkat cells, using PC Antibody. The lane on the right is blocked with the synthesized peptide.
Western blot analysis of the lysates from HT-29 cells using PC antibody.