NGEP rabbit pAb

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NGEP rabbit pAb

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ES6477

Size：	Price：
50 µL	$148.00
100 µL	$248.00

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Overview

Product name:	NGEP rabbit pAb
Reactivity:	Human;Rat;Mouse;
Alternative Names:	ANO7; NGEP; PCANAP5; TMEM16G; Anoctamin-7; Dresden transmembrane protein of the prostate; D-TMPP; IPCA-5; New gene expressed in prostate; Prostate cancer-associated protein 5; Transmembrane protein 16G
Source:	Rabbit
Dilutions:	Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.
Immunogen:	The antiserum was produced against synthesized peptide derived from human TM16G. AA range:881-930
Storage:	-20°C/1 year
Clonality:	Polyclonal
Isotype:	IgG
Concentration:	1 mg/ml
Observed Band:	105kD
GeneID:	50636
Human Swiss-Prot No:	Q6IWH7
Cellular localization:	[Isoform 1]: Cell membrane ; Multi-pass membrane protein . Cell junction . Endoplasmic reticulum . Concentrates at sites of cell-cell contact (PubMed:17308099). Shows an intracellular localization according to PubMed:22075693 and PubMed:20056604. .; [Isoform 2]: Cytoplasm, cytosol .
Background:	This prostate-specific gene encodes a cytoplasmic protein, as well as a polytopic membrane protein which may serve as a target in prostate cancer diagnosis and immunotherapy. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Aug 2011],

Immunofluorescence analysis of Hela cell. 1,NGEP Polyclonal Antibody(green) was diluted at 1:200(4° overnight). 2, Goat Anti Rabbit Alexa Fluor 488 Catalog:RS3211 was diluted at 1:1000(room temperature, 50min). 3 DAPI(blue) 10min.
Western Blot analysis of 3T3 cells using NGEP Polyclonal Antibody diluted at 1:1000
Immunofluorescence analysis of HepG2 cells, using TM16G Antibody. The picture on the right is blocked with the synthesized peptide.
Immunohistochemistry analysis of paraffin-embedded human prostate carcinoma tissue, using TM16G Antibody. The picture on the right is blocked with the synthesized peptide.