VE-Cadherin rabbit pAb

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VE-Cadherin rabbit pAb

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ES4341

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50 µL	$148.00
100 µL	$248.00

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Overview

Product name:	VE-Cadherin rabbit pAb
Reactivity:	Human;Mouse;Rat
Alternative Names:	CDH5; Cadherin-5; 7B4 antigen; Vascular endothelial cadherin; VE-cadherin; CD144
Source:	Rabbit
Dilutions:	IF: 1:50-200 WB 1:500-2000, ELISA 1:10000-20000 IHC 1:50-300
Immunogen:	The antiserum was produced against synthesized peptide derived from the Internal region of human CDH5. AA range:391-440
Storage:	-20°C/1 year
Clonality:	Polyclonal
Isotype:	IgG
Concentration:	1 mg/ml
Observed Band:	86kD
GeneID:	1003
Human Swiss-Prot No:	P33151
Cellular localization:	Cell junction . Cell membrane ; Single-pass type I membrane protein . Found at cell-cell boundaries and probably at cell-matrix boundaries. KRIT1 and CDH5 reciprocally regulate their localization to endothelial cell-cell junctions. .
Background:	This gene encodes a classical cadherin of the cadherin superfamily. The encoded preproprotein is proteolytically processed to generate the mature glycoprotein. This calcium-dependent cell-cell adhesion molecule is comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Functioning as a classical cadherin by imparting to cells the ability to adhere in a homophilic manner, this protein plays a role in endothelial adherens junction assembly and maintenance. This gene is located in a gene cluster in a region on the long arm of chromosome 16 that is involved in loss of heterozygosity events in breast and prostate cancer. [provided by RefSeq, Nov 2015],

Immunofluorescence analysis of A549. 1,primary Antibody(red) was diluted at 1:200(4°C overnight). 2, Goat Anti Rabbit IgG (H&L) - Alexa Fluor 594 Secondary antibody was diluted at 1:1000(room temperature, 50min).3, Picture B: DAPI(blue) 10min.
Western blot analysis of lysates from 1) Hela, 2) mouse-lung ,3) mouse-kidney cells, （Green） primary antibody was diluted at 1:1000, 4°over night, secondary antibody(cat:RS23920)was diluted at 1:10000, 37° 1hour. （Red） GAPDH Monoclonal Antibody(2B8) (ca
Immunofluorescence analysis of human-lung tissue. 1,VE-Cadherin Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target.
Immunofluorescence analysis of human-lung tissue. 1,VE-Cadherin Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target.