ZIS rabbit pAb

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ZIS rabbit pAb

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ES3732

Size：	Price：
50 µL	$148.00
100 µL	$248.00

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Overview

Product name:	ZIS rabbit pAb
Reactivity:	Human;Mouse;Rat
Alternative Names:	ZRANB2; ZIS; ZNF265; Zinc finger Ran-binding domain-containing protein 2; Zinc finger protein 265; Zinc finger; splicing
Source:	Rabbit
Dilutions:	Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications.
Immunogen:	The antiserum was produced against synthesized peptide derived from human ZNF265. AA range:106-155
Storage:	-20°C/1 year
Clonality:	Polyclonal
Isotype:	IgG
Concentration:	1 mg/ml
Observed Band:	34kD
GeneID:	9406
Human Swiss-Prot No:	O95218
Cellular localization:	Nucleus .
Background:	domain:The RanBP2-type zinc fingers mediate binding to RNA.,function:Splice factor required for alternative splicing of TRA2B/SFRS10 transcripts. May interfere with constitutive 5'-splice site selection.,PTM:Isoform 2 is phosphorylated on Ser-310 upon DNA damage, probably by ATM or ATR.,sequence caution:Intron retention.,similarity:Belongs to the ZRANB2 family.,similarity:Contains 2 RanBP2-type zinc fingers.,subunit:Interacts with the C-terminal half of SNRNP70, the Arg/Ser-rich domain of XE7 as well as with U2AF1 and CLK1.,

Western Blot analysis of various cells using ZIS Polyclonal Antibody. Secondary antibody(catalog#:RS0002) was diluted at 1:20000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA).
Immunofluorescence analysis of HUVEC cells, using ZNF265 Antibody. The picture on the right is blocked with the synthesized peptide.
Western blot analysis of lysates from HeLa cells, using ZNF265 Antibody. The lane on the right is blocked with the synthesized peptide.
Immunohistochemical analysis of paraffin-embedded human oophoroma. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).