SnoN rabbit pAb

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SnoN rabbit pAb

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ES3473

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50 µL	$148.00
100 µL	$248.00

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Overview

Product name:	SnoN rabbit pAb
Reactivity:	Human;Mouse
Alternative Names:	SKIL; SNO; Ski-like protein; Ski-related oncogene; Ski-related protein
Source:	Rabbit
Dilutions:	Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/40000. Not yet tested in other applications.
Immunogen:	The antiserum was produced against synthesized peptide derived from human SKIL. AA range:616-665
Storage:	-20°C/1 year
Clonality:	Polyclonal
Isotype:	IgG
Concentration:	1 mg/ml
Observed Band:	77kD
GeneID:	6498
Human Swiss-Prot No:	P12757
Cellular localization:	acrosomal vesicle,nucleus,nucleoplasm,transcription factor complex,cytoplasm,PML body,transcriptional repressor complex,protein complex,
Background:	The protein encoded by this gene is a component of the SMAD pathway, which regulates cell growth and differentiation through transforming growth factor-beta (TGFB). In the absence of ligand, the encoded protein binds to the promoter region of TGFB-responsive genes and recruits a nuclear repressor complex. TGFB signaling causes SMAD3 to enter the nucleus and degrade this protein, allowing these genes to be activated. Four transcript variants encoding three different isoforms have been found for this gene. [provided by RefSeq, Oct 2011],

Western Blot analysis of various cells using SnoN Polyclonal Antibody diluted at 1:1000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA).
Western blot analysis of lysates from HUVEC and Jurkat cells, using SKIL Antibody. The lane on the right is blocked with the synthesized peptide.
Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).