Ribosomal Protein L35 rabbit pAb

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Ribosomal Protein L35 rabbit pAb

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ES3366

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50 µL	$148.00
100 µL	$248.00

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Overview

Product name:	Ribosomal Protein L35 rabbit pAb
Reactivity:	Human;Mouse;Rat
Alternative Names:	RPL35; 60S ribosomal protein L35
Source:	Rabbit
Dilutions:	Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/10000. Not yet tested in other applications.
Immunogen:	The antiserum was produced against synthesized peptide derived from human RPL35. AA range:51-100
Storage:	-20°C/1 year
Clonality:	Polyclonal
Isotype:	IgG
Concentration:	1 mg/ml
Observed Band:	18kD
GeneID:	11224
Human Swiss-Prot No:	P42766
Cellular localization:	nucleolus,cytoplasm,cytosol,ribosome,membrane,cytosolic large ribosomal subunit,
Background:	Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L29P family of ribosomal proteins. It is located in the cytoplasm. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq, Jul 2008],

Western Blot analysis of various cells using Ribosomal Protein L35 Polyclonal Antibody diluted at 1:2000
Western blot analysis of lysates from 293, HeLa, COLO, and Jurkat cells, using RPL35 Antibody. The lane on the right is blocked with the synthesized peptide.
Western blot analysis of the lysates from HeLa cells using RPL35 antibody.
Immunohistochemical analysis of paraffin-embedded human brain tumor. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).