Western Blot analysis of various cells using Pitx1 Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA).

Western Blot analysis of Jurkat cells using Pitx1 Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA).

Western blot analysis of lysates from Jurkat and COLO cells, using PITX1 Antibody. The lane on the right is blocked with the synthesized peptide.

Immunohistochemical analysis of paraffin-embedded human spleen. 1, Tris-EDTA,pH9.0 was used for antigen retrieval. 2 Antibody was diluted at 1:200(4° overnight.3,Secondary antibody was diluted at 1:200(room temperature, 45min).