OPN rabbit pAb

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OPN rabbit pAb

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ES3083

Size：	Price：
50 µL	$148.00
100 µL	$248.00

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Overview

Product name:	OPN rabbit pAb
Reactivity:	Human;Mouse;Rat
Alternative Names:	SPP1; BNSP; OPN; PSEC0156; Osteopontin; Bone sialoprotein 1; Nephropontin; Secreted phosphoprotein 1; SPP-1; Urinary stone protein; Uropontin
Source:	Rabbit
Dilutions:	WB 1:500-2000;IHC-p 1:100-500;IF/ICC 1:100-500;ELISA 1:5000-20000
Immunogen:	The antiserum was produced against synthesized peptide derived from human Osteopontin. AA range:261-310
Storage:	-20°C/1 year
Clonality:	Polyclonal
Isotype:	IgG
Concentration:	1 mg/ml
Observed Band:	60kD
GeneID:	6696
Human Swiss-Prot No:	P10451
Cellular localization:	Secreted.
Background:	secreted phosphoprotein 1(SPP1) Homo sapiens The protein encoded by this gene is involved in the attachment of osteoclasts to the mineralized bone matrix. The encoded protein is secreted and binds hydroxyapatite with high affinity. The osteoclast vitronectin receptor is found in the cell membrane and may be involved in the binding to this protein. This protein is also a cytokine that upregulates expression of interferon-gamma and interleukin-12. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2011],

Western Blot analysis of various cells using OPN Polyclonal Antibody diluted at 1:500
Western Blot analysis of 453 cells using OPN Polyclonal Antibody diluted at 1:500
Western blot analysis of lysates from LOVO cells, using Osteopontin Antibody. The lane on the right is blocked with the synthesized peptide.
Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).