MLH1 rabbit pAb

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MLH1 rabbit pAb

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ES2797

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50 µL	$148.00
100 µL	$248.00

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Overview

Product name:	MLH1 rabbit pAb
Reactivity:	Human;Mouse;Rat
Alternative Names:	MLH1; COCA2; DNA mismatch repair protein Mlh1; MutL protein homolog 1
Source:	Rabbit
Dilutions:	WB 1:500-2000;IHC-p 1:50-300
Immunogen:	The antiserum was produced against synthesized peptide derived from human MLH1. AA range:441-490
Storage:	-20°C/1 year
Clonality:	Polyclonal
Isotype:	IgG
Concentration:	1 mg/ml
Observed Band:	85kD
GeneID:	4292
Human Swiss-Prot No:	P40692
Cellular localization:	Nucleus . Chromosome . Recruited to chromatin in a MCM9-dependent manner. .
Background:	This gene was identified as a locus frequently mutated in hereditary nonpolyposis colon cancer (HNPCC). It is a human homolog of the E. coli DNA mismatch repair gene mutL, consistent with the characteristic alterations in microsatellite sequences (RER+phenotype) found in HNPCC. Alternative splicing results in multiple transcript variants encoding distinct isoforms. Additional transcript variants have been described, but their full-length natures have not been determined.[provided by RefSeq, Nov 2009],

Western Blot analysis of various cells using MLH1 Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA).
Western Blot analysis of Jurkat cells using MLH1 Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA).
Western blot analysis of lysates from K562 cells, using MLH1 Antibody. The lane on the right is blocked with the synthesized peptide.
Immunohistochemical analysis of paraffin-embedded human Colon cancer. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).