MBD3 rabbit pAb

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MBD3 rabbit pAb

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ES2756

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50 µL	$148.00
100 µL	$248.00

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Overview

Product name:	MBD3 rabbit pAb
Reactivity:	Human;Mouse
Alternative Names:	MBD3; Methyl-CpG-binding domain protein 3; Methyl-CpG-binding protein MBD3
Source:	Rabbit
Dilutions:	WB 1:500-2000;IHC-p 1:50-300; ELISA 2000-20000
Immunogen:	The antiserum was produced against synthesized peptide derived from human MBD3. AA range:221-270
Storage:	-20°C/1 year
Clonality:	Polyclonal
Isotype:	IgG
Concentration:	1 mg/ml
Observed Band:	33kD
GeneID:	53615
Human Swiss-Prot No:	O95983
Cellular localization:	Nucleus. Chromosome. Nuclear, in discrete foci. Detected on chromatin, at promoter regions of active genes.
Background:	DNA methylation is the major modification of eukaryotic genomes and plays an essential role in mammalian development. This gene belongs to a family of nuclear proteins which are characterized by the presence of a methyl-CpG binding domain (MBD). The encoded protein is a subunit of the NuRD, a multisubunit complex containing nucleosome remodeling and histone deacetylase activities. Unlike the other family members, the encoded protein is not capable of binding to methylated DNA. The protein mediates the association of metastasis-associated protein 2 with the core histone deacetylase complex. Alternative splicing results in multiple transcript variants of this gene. [provided by RefSeq, Jul 2013],

Western Blot analysis of various cells using MBD3 Polyclonal Antibody
Western blot analysis of lysates from Jurkat cells, using MBD3 Antibody. The lane on the right is blocked with the synthesized peptide.
Immunohistochemical analysis of paraffin-embedded human liver cancer. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).