HRI rabbit pAb

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HRI rabbit pAb

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ES2566

Size：	Price：
50 µL	$148.00
100 µL	$248.00

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Overview

Product name:	HRI rabbit pAb
Reactivity:	Human;Mouse;Monkey
Alternative Names:	EIF2AK1; HRI; KIAA1369; Eukaryotic translation initiation factor 2-alpha kinase 1; Heme-controlled repressor; HCR; Heme-regulated eukaryotic initiation factor eIF-2-alpha kinase; Heme-regulated inhibitor; Hemin-sensitive initiation factor 2
Source:	Rabbit
Dilutions:	Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. IF 1:100-300 Not yet tested in other applications.
Immunogen:	The antiserum was produced against synthesized peptide derived from human EIF2AK1. AA range:571-620
Storage:	-20°C/1 year
Clonality:	Polyclonal
Isotype:	IgG
Concentration:	1 mg/ml
Observed Band:	71kD
GeneID:	27102
Human Swiss-Prot No:	Q9BQI3
Cellular localization:	cytoplasm,
Background:	The protein encoded by this gene acts at the level of translation initiation to downregulate protein synthesis in response to stress. The encoded protein is a kinase that can be inactivated by hemin. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2008],

Immunofluorescence analysis of A549. 1,primary Antibody(red) was diluted at 1:200(4°C overnight). 2, Goat Anti Rabbit IgG (H&L) - Alexa Fluor 594 Secondary antibody was diluted at 1:1000(room temperature, 50min).3, Picture B: DAPI(blue) 10min.
Western Blot analysis of various cells using HRI Polyclonal Antibody
Western blot analysis of lysates from A549 and COS7 cells, using EIF2AK1 Antibody. The lane on the right is blocked with the synthesized peptide.
Western blot analysis of the lysates from COLO205 cells using EIF2AK1 antibody.