HMG-2 rabbit pAb

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HMG-2 rabbit pAb

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ES2540

Size：	Price：
50 µL	$148.00
100 µL	$248.00

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Overview

Product name:	HMG-2 rabbit pAb
Reactivity:	Human;Mouse;Rat
Alternative Names:	HMGB2; HMG2; High mobility group protein B2; High mobility group protein 2; HMG-2
Source:	Rabbit
Dilutions:	Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications.
Immunogen:	The antiserum was produced against synthesized peptide derived from human HMGB2. AA range:131-180
Storage:	-20°C/1 year
Clonality:	Polyclonal
Isotype:	IgG
Concentration:	1 mg/ml
Observed Band:	27kD
GeneID:	3148
Human Swiss-Prot No:	P26583
Cellular localization:	Nucleus . Chromosome . Cytoplasm . Secreted . In basal state predominantly nuclear. .
Background:	This gene encodes a member of the non-histone chromosomal high mobility group protein family. The proteins of this family are chromatin-associated and ubiquitously distributed in the nucleus of higher eukaryotic cells. In vitro studies have demonstrated that this protein is able to efficiently bend DNA and form DNA circles. These studies suggest a role in facilitating cooperative interactions between cis-acting proteins by promoting DNA flexibility. This protein was also reported to be involved in the final ligation step in DNA end-joining processes of DNA double-strand breaks repair and V(D)J recombination. [provided by RefSeq, Jul 2008],

Western Blot analysis of various cells using HMG-2 Polyclonal Antibody
Western blot analysis of lysates from COLO205 cells, using HMGB2 Antibody. The lane on the right is blocked with the synthesized peptide.
Immunohistochemical analysis of paraffin-embedded human liver cancer. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).