Rac1/CDC42 (Phospho Ser71) rabbit pAb

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Rac1/CDC42 (Phospho Ser71) rabbit pAb

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ES20202

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50 µL	$148.00
100 µL	$248.00

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Overview

Product name:	Rac1/CDC42 (Phospho Ser71) rabbit pAb
Reactivity:	Human;Mouse;Rat
Alternative Names:	Ras-related C3 botulinum toxin substrate 1 (Cell migration-inducing gene 5 protein;Ras-like protein TC25;p21-Rac1)
Source:	Rabbit
Dilutions:	WB 1:500-2000;IHC-p 1:50-300; ELISA 2000-20000
Immunogen:	Synthesized peptide derived from human Rac1/CDC42 (Phospho Ser71)
Storage:	-20°C/1 year
Clonality:	Polyclonal
Isotype:	IgG
Concentration:	1 mg/ml
Molecular Weight:	21kD
GeneID:	5879
Human Swiss-Prot No:	P63000/P15153/P60763/P60953
Cellular localization:	Cell membrane ; Lipid-anchor ; Cytoplasmic side . Melanosome . Cytoplasm . Cell projection, lamellipodium . Cell projection, dendrite . Cell junction, synapse . Nucleus . Inner surface of plasma membrane possibly with attachment requiring prenylation of the C-terminal cysteine (PubMed:1903399). Identified by mass spectrometry in melanosome fractions from stage I to stage IV (PubMed:17081065). Found in the ruffled border (a late endosomal-like compartment in the plasma membrane) of bone-resorbing osteoclasts. Localizes to the lamellipodium in a SH3RF1-dependent manner (By similarity). In macrophages, cytoplasmic location increases upon CSF1 stimulation (By similarity). Activation by GTP-binding promotes nuclear localization (PubMed:12551911). .

Western blot analysis of Rac1(Phospho-Ser71) antibody in 293 cells lysates, cell treated or untreated with PMA 125ng/ml 30', 4° over night, secondary antibody(cat: RS0002 was diluted at 1:10000, 37° 1hour.
Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using Rac1(Phospho-Ser71) antibody
Immunohistochemical analysis of paraffin-embedded human uterus. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).
Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).