Smad1 (Phospho Ser214) rabbit pAb

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Smad1 (Phospho Ser214) rabbit pAb

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ES20159

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50 µL	$148.00
100 µL	$248.00

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Overview

Product name:	Smad1 (Phospho Ser214) rabbit pAb
Reactivity:	Human;Mouse;Rat
Alternative Names:	Mothers against decapentaplegic homolog 1 (MAD homolog 1;Mothers against DPP homolog 1;JV4-1;Mad-related protein 1;SMAD family member 1;SMAD 1;Smad1;hSMAD1;Transforming growth factor-beta-signaling protein 1;BSP-1)
Source:	Rabbit
Dilutions:	WB 1:500-2000;IHC-p 1:50-300
Immunogen:	Synthesized peptide derived from human Smad1 (Phospho Ser214)
Storage:	-20°C/1 year
Clonality:	Polyclonal
Isotype:	IgG
Concentration:	1 mg/ml
Observed Band:	51kD
GeneID:	4086
Human Swiss-Prot No:	Q15797
Cellular localization:	Cytoplasm . Nucleus . Cytoplasmic in the absence of ligand. Migrates to the nucleus when complexed with SMAD4 (PubMed:15647271). Co-localizes with LEMD3 at the nucleus inner membrane (PubMed:15647271). Exported from the nucleus to the cytoplasm when dephosphorylated (By similarity). .
Background:	function:Transcriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase. SMAD1 is a receptor-regulated SMAD (R-SMAD).,PTM:Phosphorylated on serine by BMP type 1 receptor kinase.,PTM:Ubiquitin-mediated proteolysis by SMAD-specific E3 ubiquitin ligase SMURF1.,similarity:Belongs to the dwarfin/SMAD family.,similarity:Contains 1 MH1 (MAD homology 1) domain.,similarity:Contains 1 MH2 (MAD homology 2) domain.,subcellular location:Cytoplasmic in the absence of ligand. Migrates to the nucleus when complexed with SMAD4.,subunit:Interacts with HGS, NANOG and ZCCHC12 (By similarity). May form trimers with another SMAD1 and the co-SMAD SMAD4. Interacts with PEBP2-alpha subunit, CREB-binding protein (CBP), p300, SMURF1, SMURF2 and HOXC8. Associates with ZNF423 or ZNF521 in response to BMP2 leading to activate transcription of BMP target genes. Interacts with LBXCOR1.,tissue specificity:Ubiquitous. Highest expression seen in the heart and skeletal muscle.,

Immunohistochemical analysis of paraffin-embedded human spleen. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).
Western Blot analysis of various,using primary antibody at 1:1000 dilution. Secondary antibody(catalog#:RS23920) was diluted at 1:10000