AF-10 rabbit pAb

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AF-10 rabbit pAb

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ES1609

Size：	Price：
50 µL	$148.00
100 µL	$248.00

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Overview

Product name:	AF-10 rabbit pAb
Reactivity:	Human;Mouse
Alternative Names:	MLLT10; AF10; Protein AF-10; ALL1-fused gene from chromosome 10 protein
Source:	Rabbit
Dilutions:	Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications.
Immunogen:	The antiserum was produced against synthesized peptide derived from human AF10. AA range:211-260
Storage:	-20°C/1 year
Clonality:	Polyclonal
Isotype:	IgG
Concentration:	1 mg/ml
Observed Band:	109kD
GeneID:	8028
Human Swiss-Prot No:	P55197
Cellular localization:	Nucleus .
Background:	myeloid/lymphoid or mixed-lineage leukemia; translocated to, 10(MLLT10) Homo sapiens This gene encodes a transcription factor and has been identified as a partner gene involved in several chromosomal rearrangements resulting in various leukemias. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2010],

Immunofluorescence analysis of Hela cell. 1,AF-10 Polyclonal Antibody(red) was diluted at 1:200(4° overnight). COX IV Monoclonal Antibody(6C8)(green) was diluted at 1:200(4° overnight). 2, Goat Anti Rabbit Alexa Fluor 594 Catalog:RS3611 was diluted at 1:1
Western Blot analysis of various cells using AF-10 Polyclonal Antibody diluted at 1:1000
Immunohistochemical analysis of paraffin-embedded Human breast cancer. Antibody was diluted at 1:100(4° overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absor
Western blot analysis of lysates from COLO205 and Jurkat cells, using AF10 Antibody. The lane on the right is blocked with the synthesized peptide.