14-3-3 θ rabbit pAb

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14-3-3 θ rabbit pAb

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ES1544

Size：	Price：
50 µL	$148.00
100 µL	$248.00

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Overview

Product name:	14-3-3 θ rabbit pAb
Reactivity:	Human;Mouse;Rat
Alternative Names:	YWHAQ; 14-3-3 protein theta; 14-3-3 protein T-cell; 14-3-3 protein tau; Protein HS1
Source:	Rabbit
Dilutions:	Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.
Immunogen:	The antiserum was produced against synthesized peptide derived from human 14-3-3 thet. AA range:41-90
Storage:	-20°C/1 year
Clonality:	Polyclonal
Isotype:	IgG
Concentration:	1 mg/ml
Observed Band:	28kD
GeneID:	10971
Human Swiss-Prot No:	P27348
Cellular localization:	Cytoplasm. In neurons, axonally transported to the nerve terminals.
Background:	This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 99% identical to the mouse and rat orthologs. This gene is upregulated in patients with amyotrophic lateral sclerosis. It contains in its 5' UTR a 6 bp tandem repeat sequence which is polymorphic, however, there is no correlation between the repeat number and the disease. [provided by RefSeq, Jul 2008],

Western Blot analysis of various cells using 14-3-3 θ Polyclonal Antibody diluted at 1:1000
Western blot analysis of lysates from HUVEC and HeLa cells, using 14-3-3 thet Antibody. The lane on the right is blocked with the synthesized peptide.
Immunohistochemical analysis of paraffin-embedded human liver cancer. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).