NF-E4 rabbit pAb

Products

NF-E4 rabbit pAb

One-click to copy product information

ES14508

Size：	Price：
50 µL	$148.00
100 µL	$248.00

Manual Send email to us

Overview

Product name:	NF-E4 rabbit pAb
Reactivity:	Human;Rat;Mouse;
Alternative Names:	Transcription factor NF-E4
Source:	Rabbit
Dilutions:	IHC-p 1:50-200, WB 1:500-2000
Immunogen:	Synthesized peptide derived from human NF-E4
Storage:	-20°C/1 year
Clonality:	Polyclonal
Isotype:	IgG
Concentration:	1 mg/ml
Observed Band:	19kD
GeneID:	58160
Human Swiss-Prot No:	Q86UQ8
Cellular localization:	Nucleus .
Background:	The erythroid-specific protein encoded by this gene, and the ubiquitous transcription factor CP2, form the stage selector protein (SSP) complex, which is involved in preferential expression of the gamma-globin genes in fetal erythroid cells. Alternate use of an in-frame upstream non-AUG (CUG) translation initiation codon, and a downstream AUG codon, results in two isoforms. While the long isoform (22 kDa) acts as an activator, the short isoform (14 kDa) has been shown to repress gamma-globin gene expression. This gene is located in an intron of the FBXL13 gene on the opposite strand. [provided by RefSeq, Jul 2008],

Western blot analysis of lysates from K562 cells, primary antibody was diluted at 1:1000, 4°over night
Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).