eNOS (phospho Ser1177) rabbit pAb

Products

eNOS (phospho Ser1177) rabbit pAb

One-click to copy product information

ES1442

Size：	Price：
50 µL	$148.00
100 µL	$248.00

Manual Send email to us

Overview

Product name:	eNOS (phospho Ser1177) rabbit pAb
Reactivity:	Human;Mouse;Rat
Alternative Names:	NOS3; Nitric oxide synthase; endothelial; Constitutive NOS; cNOS; EC-NOS; Endothelial NOS; eNOS; NOS type III; NOSIII
Source:	Rabbit
Dilutions:	Western Blot: 1/500 - 1/2000. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.
Immunogen:	The antiserum was produced against synthesized peptide derived from human eNOS around the phosphorylation site of Ser1176. AA range:1144-1193
Storage:	-20°C/1 year
Clonality:	Polyclonal
Isotype:	IgG
Concentration:	1 mg/ml
Observed Band:	130-140kD
GeneID:	4846
Human Swiss-Prot No:	P29474
Cellular localization:	Cell membrane. Membrane, caveola. Cytoplasm, cytoskeleton. Golgi apparatus. Specifically associates with actin cytoskeleton in the G2 phase of the cell cycle; which is favored by interaction with NOSIP and results in a reduced enzymatic activity.
Background:	Nitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. Nitric oxide is synthesized from L-arginine by nitric oxide synthases. Variations in this gene are associated with susceptibility to coronary spasm. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2009],

Immunofluorescence analysis of A549. 1,primary Antibody(red) was diluted at 1:200(4°C overnight). 2, Goat Anti Rabbit IgG (H&L) - Alexa Fluor 594 Secondary antibody was diluted at 1:1000(room temperature, 50min).3, Picture B: DAPI(blue) 10min.
Immunofluorescence analysis of Hela cell. 1,NOS3 (phospho Ser1177) Polyclonal Antibody(green) was diluted at 1:200(4° overnight). 2, Goat Anti Rabbit Alexa Fluor 488 Catalog:RS3211 was diluted at 1:1000(room temperature, 50min). 3 DAPI(blue) 10min.
Western Blot analysis of various cells using Phospho-NOS3 (S1177) Polyclonal Antibody diluted at 1:1000
Western Blot analysis of HuvEc cells using Phospho-NOS3 (S1177) Polyclonal Antibody diluted at 1:1000