PHF5A rabbit pAb

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PHF5A rabbit pAb

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ES14138

Size：	Price：
50 µL	$148.00
100 µL	$248.00

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Overview

Product name:	PHF5A rabbit pAb
Reactivity:	Human; Mouse;Rat
Source:	Rabbit
Dilutions:	WB 1:500-2000;IHC-p 1:50-300; ELISA 2000-20000
Immunogen:	Synthesized peptide derived from human PHF5A AA range: 33-83
Storage:	-20°C/1 year
Clonality:	Polyclonal
Isotype:	IgG
Concentration:	1 mg/ml
Molecular Weight:	12kD
GeneID:	84844
Human Swiss-Prot No:	Q7RTV0
Cellular localization:	Nucleus . Nucleus speckle .
Background:	This gene encodes a subunit of the splicing factor 3b protein complex. Splicing factor 3b, together with splicing factor 3a and a 12S RNA unit, forms the U2 small nuclear ribonucleoproteins complex (U2 snRNP). The splicing factor 3b/3a complex binds pre-mRNA upstream of the intron's branch site in a sequence-independent manner and may anchor the U2 snRNP to the pre-mRNA. The protein encoded by this gene contains a PHD-finger-like domain that is flanked by highly basic N- and C-termini. This protein belongs to the PHD-finger superfamily and may act as a chromatin-associated protein. This gene has several pseudogenes on different chromosomes. [provided by RefSeq, Jul 2008],

Western blot analysis of lysates from AD293 cells, primary antibody was diluted at 1:1000, 4°over night
Immunohistochemical analysis of paraffin-embedded human liver cancer. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).