Sp1 (phospho Thr739) rabbit pAb

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Sp1 (phospho Thr739) rabbit pAb

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ES1403

Size：	Price：
50 µL	$148.00
100 µL	$248.00

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Overview

Product name:	Sp1 (phospho Thr739) rabbit pAb
Reactivity:	Human;Mouse;Rat;Monkey
Alternative Names:	SP1; TSFP1; Transcription factor Sp1
Source:	Rabbit
Dilutions:	Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/5000. Not yet tested in other applications.
Immunogen:	The antiserum was produced against synthesized peptide derived from human SP1 around the phosphorylation site of Thr739. AA range:706-755
Storage:	-20°C/1 year
Clonality:	Polyclonal
Isotype:	IgG
Concentration:	1 mg/ml
Observed Band:	81kD
GeneID:	6667
Human Swiss-Prot No:	P08047
Cellular localization:	Nucleus. Cytoplasm. Nuclear location is governed by glycosylated/phosphorylated states. Insulin promotes nuclear location, while glucagon favors cytoplasmic location.
Background:	The protein encoded by this gene is a zinc finger transcription factor that binds to GC-rich motifs of many promoters. The encoded protein is involved in many cellular processes, including cell differentiation, cell growth, apoptosis, immune responses, response to DNA damage, and chromatin remodeling. Post-translational modifications such as phosphorylation, acetylation, glycosylation, and proteolytic processing significantly affect the activity of this protein, which can be an activator or a repressor. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2014],

Western Blot analysis of various cells using Phospho-Sp1 (T739) Polyclonal Antibody diluted at 1:1000
Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using SP1 (Phospho-Thr739) Antibody
Western blot analysis of lysates from COS7 cells treated with serum 20% 15', using SP1 (Phospho-Thr739) Antibody. The lane on the right is blocked with the phospho peptide.
Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).