PRAS40 (phospho-Ser183) rabbit pAb

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PRAS40 (phospho-Ser183) rabbit pAb

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ES13968

Size：	Price：
50 µL	$148.00
100 µL	$248.00

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Overview

Product name:	PRAS40 (phospho-Ser183) rabbit pAb
Reactivity:	Human;Mouse
Alternative Names:	Proline-rich AKT1 substrate 1 (40 kDa proline-rich AKT substrate)
Source:	Rabbit
Dilutions:	WB 1:500-2000;IHC-p 1:50-300; ELISA 2000-20000
Immunogen:	Synthesized phosho peptide around human PRAS40 (Ser183)
Storage:	-20°C/1 year
Clonality:	Polyclonal
Isotype:	IgG
Concentration:	1 mg/ml
Observed Band:	40kD
GeneID:	84335
Human Swiss-Prot No:	Q96B36
Cellular localization:	Cytoplasm, cytosol . Found in the cytosolic fraction of the brain. .
Background:	AKT1S1 is a proline-rich substrate of AKT (MIM 164730) that binds 14-3-3 protein (see YWHAH, MIM 113508) when phosphorylated (Kovacina et al., 2003 [PubMed 12524439]).[supplied by OMIM, Mar 2008],

Western blot analysis of lysates from U2OS cells, primary antibody was diluted at 1:1000, 4°over night
Immunohistochemical analysis of paraffin-embedded human Squamous cell carcinoma of lung. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).