PUS10 rabbit pAb

Products

PUS10 rabbit pAb

One-click to copy product information

ES13856

Size：	Price：
50 µL	$148.00
100 µL	$248.00

Manual Send email to us

Overview

Product name:	PUS10 rabbit pAb
Reactivity:	Human; Mouse
Source:	Rabbit
Dilutions:	WB 1:500-2000;IHC-p 1:50-300; ELISA 2000-20000
Immunogen:	Synthesized peptide derived from human PUS10 AA range: 425-475
Storage:	-20°C/1 year
Clonality:	Polyclonal
Isotype:	IgG
Concentration:	1 mg/ml
Molecular Weight:	58kD
GeneID:	150962
Human Swiss-Prot No:	Q3MIT2
Cellular localization:	Nucleus . Cytoplasm . Mitochondrion . Localizes mainly in the nucleus (Probable) (PubMed:31819270). tRNA pseudouridylate synthase activity is restricted to the cytoplasm (PubMed:31819270). Translocates from nucleus to mitochondria during TRAIL-induced apoptosis (PubMed:28981101). .
Background:	Pseudouridination, the isomerization of uridine to pseudouridine, is the most common posttranscriptional nucleotide modification found in RNA and is essential for biologic functions such as spliceosome biogenesis. Pseudouridylate synthases, such as PUS10, catalyze pseudouridination of structural RNAs, including transfer, ribosomal, and splicing RNAs. These enzymes also act as RNA chaperones, facilitating the correct folding and assembly of tRNAs (McCleverty et al., 2007 [PubMed 17900615]).[supplied by OMIM, May 2009],

Western blot analysis of lysates from THP-1 cells, primary antibody was diluted at 1:1000, 4°over night
Immunohistochemical analysis of paraffin-embedded human cervical carcinoma. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).