RASF5 rabbit pAb

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RASF5 rabbit pAb

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ES13460

Size：	Price：
50 µL	$148.00
100 µL	$248.00

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Overview

Product name:	RASF5 rabbit pAb
Reactivity:	Human; Mouse;Rat
Source:	Rabbit
Dilutions:	WB 1:500-2000; IF/ICC 1:50-200;ELISA 1:2000-20000;IHC-p 1:50-200
Immunogen:	Synthesized peptide derived from human RASF5 AA range: 234-284
Storage:	-20°C/1 year
Clonality:	Polyclonal
Isotype:	IgG
Concentration:	1 mg/ml
Molecular Weight:	46kD
GeneID:	83593
Human Swiss-Prot No:	Q8WWW0
Cellular localization:	Cytoplasm. Cytoplasm, cytoskeleton. Isoform 2 is mainly located in the perinuclear region of unstimulated primary T-cells. Upon stimulation translocates to the leading edge and colocalizes with ITGAL/LFA-1 in the peripheral zone of the immunological synapse. Isoform 2 is localized to growing microtubules in vascular endothelial cells and is dissociated from microtubules by activated RAP1A.
Background:	This gene is a member of the Ras association domain family. It functions as a tumor suppressor, and is inactivated in a variety of cancers. The encoded protein localizes to centrosomes and microtubules, and associates with the GTP-activated forms of Ras, Rap1, and several other Ras-like small GTPases. The protein regulates lymphocyte adhesion and suppresses cell growth in response to activated Rap1 or Ras. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008],

Western blot analysis of lysates from Hela cells, primary antibody was diluted at 1:1000, 4°over night
Immunohistochemical analysis of paraffin-embedded human uterus. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).