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DDDDK-Tag(binds to flag sequnence) rabbit pAb
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ES1078
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50 µL $148.00
100 µL $248.00
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Overview

Product name: DDDDK-Tag(binds to flag sequnence) rabbit pAb
Reactivity: Species independent
Alternative Names: DDDDK epitope tag; DDDDK epitope tag; DYKDDDDK epitope tag
Source: Rabbit
Dilutions: Western Blot: 1/1000 - 1/3000. ELISA: 1:5000-20000. Not yet tested in other applications.
Immunogen: DDDDK synthetic peptide conjugated to KLH.
Storage: -20°C/1 year
Clonality: Polyclonal
Isotype: IgG
Concentration: 1 mg/ml
Background: The DYKDDDDK (FLAG) peptide has been used extensively as a general tag in expression vectors. This peptide can be expressed and detected with the protein of interest as an amino-terminal or carboxy-terminal fusion. N-terminal FLAG vectors provide an Ek cleavage site for removal of the fusion tag. The FLAG peptide is likely to be located on the surface of a fusion protein because of its hydrophilic nature. As a result, the FLAG peptide is more likely to be accessible to antibodies. A FLAG-tag can be used in many different assays that require recognition by an antibody, such as western blotting, immunocytochemistry, immunoprecipitation, flow cytometry, protein purification, and in the study of protein-protein interactions, cell ultrastructure, and protein localization and so on.