| Introduction: |
The fluorescence signal of SYBR Green Dye combined with dsDNA can be enhanced 800-1000 times. In the PCR reaction system, an excess of SYBR Green Dye fluorescent dye is added. After it is specifically incorporated into the DNA double-strand, the fluorescent signal is enhanced, while the fluorescence of the SYBR Green Dye dye molecule that is not incorporated in the chain remains unchanged, thereby ensuring the fluorescence signal. The increase is completely synchronized with the increase of PCR products. Fluorescence can be measured in the annealing stage or the extension stage. |
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