Product name: |
VSV-G-Tag Mouse mAb |
Source: |
Mouse |
Dilutions: |
WB 1:5,000 IP 1:200 IF 1:1,000 |
Immunogen: |
Synthetic Peptide |
Storage: |
PBS with 0.02% sodium azide and 50% glycerol pH 7.4. Store at -20°C. Avoid repeated freeze-thaw cycles. |
Clonality: |
Monoclonal |
Isotype: |
IgG1 |
Concentration: |
1mg/mL |
Background: |
Vesicular stomatitis virus (VSV), an enveloped RNA virus from the Rhabdoviridae family, is released from the plasma membrane of host cells by a process called budding. The fusiogenic envelope G glycoprotein of the vesicular stomatitis virus (VSV-G) that has been used to pseudotype retrovirus and lentivirus vectors can be used alone as an efficient vehicle for gene transfer. VSV-G protein is secreted into the culture medium as sendimentable vesicles from cells transfected with a VSV-G expression plasmid in the absence of other viral components. The VSV-G vesicles in the conditioned medium can be partially purified by pelleting through sucrose cushion ultracentrifugation. |