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Human IGF2BP2(Insulin-Like Growth Factor 2 mRNA-Binding Protein 2) ELISA Kit
ELK9924
Size： Price：
48 T $320.00
96 T $458.00
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Overview

Product name: Human IGF2BP2(Insulin-Like Growth Factor 2 mRNA-Binding Protein 2) ELISA Kit
Reactivity: Human
Alternative Names: IGF2 mRNA-binding protein 2; IMP-2; Hepatocellular carcinoma autoantigen p62; IGF-II mRNA-binding protein 2; VICKZ family member 2
Assay Type: Sandwich
Sensitivity: 48.9 pg/mL
Standard: 8000 pg/mL
Detection Range: 125-8000 pg/mL
Sample Type: Serum, plasma and other biological fluids.
Assay Length: 3.5h
Research Area: Tumor immunity;Endocrinology;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human IGF2BP2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human IGF2BP2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human IGF2BP2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human IGF2BP2 in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (pg/mL) OD Corrected OD
8000.00 2.172 2.077
4000.00 1.700 1.605
2000.00 1.146 1.051
1000.00 0.877 0.782
500.00 0.572 0.477
250.00 0.273 0.178
125.00 0.175 0.080
0.00 0.095 0.000

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant IGF2BP2 and the recovery rates were calculated by comparing the measured value to the expected amount of IGF2BP2 in samples.
Matrix Recovery range Average
serum(n=5) 96-109% 102%
EDTA plasma(n=5) 79-93% 86%
Heparin plasma(n=5) 80-97% 88%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of IGF2BP2 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 85-94% 87-101% 89-99% 85-95%
EDTA plasma(n=5) 88-99% 81-93% 89-97% 85-94%
Heparin plasma(n=5) 95-103% 79-97% 93-102% 87-103%