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Mouse Smad2/3 (Mothers Against Decapentaplegic Homolog 2/3) ELISA Kit
ELK9883
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48 T $320.00
96 T $458.00
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Overview

Product name: Mouse Smad2/3 (Mothers Against Decapentaplegic Homolog 2/3) ELISA Kit
Reactivity: Mouse
Alternative Names: Smad 2/3
Assay Type: Sandwich
Sensitivity: 0.059 ng/mL
Standard: 10 ng/mL
Detection Range: 0.16-10 ng/mL
Sample Type: serum, plasma, tissue homogenates
Assay Length: 3.5h
Research Area: Signal transduction;Apoptosis;Tumor immunity;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse Smad2/3. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse Smad2/3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse Smad2/3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse Smad2/3 in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (ng/mL) OD Corrected OD
10.00 2.204 2.122
5.00 1.612 1.530
2.50 1.219 1.137
1.25 0.828 0.746
0.63 0.528 0.446
0.32 0.377 0.295
0.16 0.241 0.159
0.00 0.082 0.000

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant Smad2/3 and the recovery rates were calculated by comparing the measured value to the expected amount of Smad2/3 in samples.
Matrix Recovery range Average
serum(n=5) 96-108% 102%
EDTA plasma(n=5) 80-97% 88%
Heparin plasma(n=5) 96-107% 101%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Smad2/3 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 85-100% 83-92% 87-96% 88-97%
EDTA plasma(n=5) 91-97% 87-101% 87-101% 85-97%
Heparin plasma(n=5) 92-105% 95-107% 82-96% 86-105%