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Rat PGF2α(Prostaglandin F2 Alpha) ELISA Kit
ELK9878
Size： Price：
48 T $320.00
96 T $458.00
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Overview

Product name: Rat PGF2α(Prostaglandin F2 Alpha) ELISA Kit
Reactivity: Rat
Alternative Names: 8-isoprostane; 8-iso-PGF2α
Assay Type: Competitive Inhibition
Sensitivity: 3.71 pg/mL
Standard: 800 pg/mL
Detection Range: 12.5-800 pg/mL
Sample Type: Serum, plasma, saliva and other biological fluids.
Assay Length: 2h
Research Area: Metabolic pathway;Cardiovascular biology;Hormone metabolism;
Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Rat PGF2α. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat PGF2α. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat PGF2α in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (pg/mL) OD Corrected OD
800.00 0.179
400.00 0.397
200.00 0.612
100.00 0.833
50.00 1.215
25.00 1.596
12.50 1.863
0.00 2.226

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant PGF2α and the recovery rates were calculated by comparing the measured value to the expected amount of PGF2α in samples.
Matrix Recovery range Average
serum(n=5) 78-95% 86%
EDTA plasma(n=5) 92-107% 99%
Heparin plasma(n=5) 85-98% 91%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of PGF2α and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 82-90% 92-101% 85-92% 87-103%
EDTA plasma(n=5) 89-101% 88-97% 88-104% 85-93%
Heparin plasma(n=5) 97-103% 87-98% 95-102% 81-103%