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Human F-PSA(Free ProstateSpecific Antigen) ELISA Kit
ELK9865
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48 T $320.00
96 T $458.00
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Overview

Product name: Human F-PSA(Free ProstateSpecific Antigen) ELISA Kit
Reactivity: Human
Alternative Names: F-PSA
Assay Type: Sandwich
Sensitivity: 17.31 pg/mL
Standard: 2000 pg/mL
Detection Range: 31.25-2000 pg/mL
Sample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length: 3.5h
Research Area: Tags & Cell Markers;Neuroscience;Signal Transduction;Cancer
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human F-PSA. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human F-PSA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human F-PSA, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human F-PSA in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (pg/mL) OD Corrected OD
2000.00 1.929 1.842
1000.00 1.599 1.512
500.00 1.251 1.164
250.00 0.757 0.670
125.00 0.585 0.498
62.50 0.323 0.236
31.25 0.226 0.139
0.00 0.087 0.000

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant F-PSA and the recovery rates were calculated by comparing the measured value to the expected amount of F-PSA in samples.
Matrix Recovery range Average
serum(n=5) 84-99% 91%
EDTA plasma(n=5) 80-97% 88%
Heparin plasma(n=5) 80-92% 86%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of F-PSA and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 78-92% 92-103% 82-96% 78-91%
EDTA plasma(n=5) 88-96% 87-98% 81-93% 87-101%
Heparin plasma(n=5) 79-91% 89-103% 82-95% 99-106%