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Human PI3(Elafin) ELISA Kit
ELK9848
Size： Price：
48 T $320.00
96 T $458.00
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Overview

Product name: Human PI3(Elafin) ELISA Kit
Reactivity: Human
Alternative Names: Cementoin; ELAF_HUMAN; Elafin; Elafin/Skalp; Elastase Specific Inhibitor; Elastase-specific inhibitor; ES 1; ESI; Peptidase inhibitor 3; Peptidase inhibitor 3; skin derived; PI 3; PI-3; PI3; Pre elafin; Protease inhibitor 3 skin derived; Protease inhibitor 3; skin derived (SKALP); Protease inhibitor WAP3; SKALP; Skin derived Anti leukoproteinase; Skin-derived antileukoproteinase; Trappin 2; WAP four disulfide core domain 14; WAP four disulfide core domain protein 14; WAP four-disulfide core domain protein 14; WAP3; WFDC14
Assay Type: Sandwich
Sensitivity: 2.8 pg/mL
Standard: 500 pg/mL
Detection Range: 7.82-500 pg/mL
Sample Type: Serum, plasma, tissue homogenates
Assay Length: 3.5h
Research Area: Enzyme & Kinase;
Uniprot ID: P19957
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human PI3. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human PI3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human PI3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human PI3 in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (pg/mL) OD Corrected OD
500.00 2.258 2.175
250.00 1.673 1.590
125.00 1.162 1.079
62.50 0.813 0.730
31.25 0.577 0.494
15.63 0.369 0.286
7.82 0.226 0.143
0.00 0.083 0.000

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant PI3 and the recovery rates were calculated by comparing the measured value to the expected amount of PI3 in samples.
Matrix Recovery range Average
serum(n=5) 90-103% 96%
EDTA plasma(n=5) 87-99% 93%
Heparin plasma(n=5) 85-97% 91%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of PI3 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 82-96% 93-101% 95-103% 95-103%
EDTA plasma(n=5) 87-98% 93-105% 89-97% 85-97%
Heparin plasma(n=5) 87-96% 88-97% 91-97% 81-93%