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Human L-Carnitine ELISA Kit
ELK9821
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48 T $320.00
96 T $458.00
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Overview

Product name: Human L-Carnitine ELISA Kit
Reactivity: Human
Alternative Names: L-Carnitine
Assay Type: Sandwich
Sensitivity: 0.119 ng/mL
Standard: 20 ng/mL
Detection Range: 0.32-20 ng/mL
Sample Type: serum, plasma, urine, tissue homogenates
Assay Length: 3.5h
Research Area: Biochemicals
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human L-Carnitine. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human L-Carnitine. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human L-Carnitine, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human L-Carnitine in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (ng/mL) OD Corrected OD
20.00 1.987 1.900
10.00 1.686 1.599
5.00 1.177 1.090
2.50 0.921 0.834
1.25 0.525 0.438
0.63 0.389 0.302
0.32 0.234 0.147
0.00 0.087 0.000

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant L-Carnitine and the recovery rates were calculated by comparing the measured value to the expected amount of L-Carnitine in samples.
Matrix Recovery range Average
serum(n=5) 87-95% 91%
EDTA plasma(n=5) 86-96% 91%
Heparin plasma(n=5) 79-97% 88%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of L-Carnitine and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 92-103% 81-99% 89-97% 87-98%
EDTA plasma(n=5) 92-105% 83-92% 85-96% 78-90%
Heparin plasma(n=5) 92-101% 88-102% 87-102% 85-97%