Products

Rat ATP(Adenosine Triphosphate) ELISA Kit
ELK9304
Size： Price：
48 T $265.00
96 T $376.00
Manual MSDS Send email to us

Overview

Product name: Rat ATP(Adenosine Triphosphate) ELISA Kit
Reactivity: Rat
Alternative Names: Adenosine-5'-Triphosphate
Assay Type: Competitive Inhibition
Sensitivity: 5.2 ng/mL
Standard: 1000 ng/mL
Detection Range: 15.63-1000 ng/mL
Sample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length: 2h
Research Area: Nutrition metabolism
Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Rat ATP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat ATP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat ATP in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (ng/mL) OD Corrected OD
1000.00 0.189
500.00 0.336
250.00 0.583
125.00 0.998
62.50 1.258
31.25 1.457
15.63 1.796
0.00 2.211

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant ATP and the recovery rates were calculated by comparing the measured value to the expected amount of ATP in samples.
Matrix Recovery range Average
serum(n=5) 78-92% 85%
EDTA plasma(n=5) 90-105% 97%
Heparin plasma(n=5) 88-102% 95%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ATP and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 85-101% 78-96% 79-98% 93-102%
EDTA plasma(n=5) 82-101% 93-105% 92-104% 82-96%
Heparin plasma(n=5) 87-101% 95-103% 97-105% 88-97%