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Mouse PDZK1IP1(PDZK1-interacting protein 1) ELISA Kit
ELK9218
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48 T $412.00
96 T $588.00
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Overview

Product name: Mouse PDZK1IP1(PDZK1-interacting protein 1) ELISA Kit
Reactivity: Mouse
Alternative Names: MAP17; 17 kDa membrane associated protein; DD96; Epithelial protein up regulated in carcinoma membrane associated protein 17; Membrane associated protein 17; PDZK1-interacting protein 1; Pdzk1ip1; RP1-18D14.5; SPAP;
Assay Type: Sandwich
Sensitivity: 13.3 pg/mL
Standard: 2000 pg/mL
Detection Range: 31.25-2000 pg/mL
Sample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length: 3.5h
Research Area: Cancer
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse PDZK1IP1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse PDZK1IP1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse PDZK1IP1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse PDZK1IP1 in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (pg/mL) OD Corrected OD
2000.00 2.172 2.085
1000.00 1.686 1.599
500.00 1.211 1.124
250.00 0.871 0.784
125.00 0.567 0.480
62.50 0.336 0.249
31.25 0.226 0.139
0.00 0.087 0.000

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant PDZK1IP1 and the recovery rates were calculated by comparing the measured value to the expected amount of PDZK1IP1 in samples.
Matrix Recovery range Average
serum(n=5) 89-105% 97%
EDTA plasma(n=5) 96-107% 102%
Heparin plasma(n=5) 80-92% 86%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of PDZK1IP1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 97-103% 82-94% 82-96% 88-101%
EDTA plasma(n=5) 90-99% 81-97% 82-96% 95-102%
Heparin plasma(n=5) 89-101% 93-108% 86-97% 90-101%