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Human hs-Hepc25(high sensitivity Hepcidin 25) Microsample ELISA Kit
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ELK9112MS
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48 T $320.00
96 T $458.00
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Overview

Product name: Human hs-Hepc25(high sensitivity Hepcidin 25) Microsample ELISA Kit
Reactivity: Human
Alternative Names: Hamp; HEPC; HEPC_HUMAN; Hepc20; Hepc25; HEPCIDIN; Hepcidin 20; Hepcidin 25; Hepcidin antimicrobial peptide; Hepcidin-20; Hepcidin25; HFE2; HFE2B; LEAP 1; LEAP-1; LEAP1; Liver expressed antimicrobial peptide; Liver-expressed antimicrobial peptide 1; PLTR; Putative liver tumor regressor;
Assay Type: Sandwich
Sensitivity: 0.61 ng/mL
Standard: 100 ng/mL
Detection Range: 1.57-100 ng/mL
Sample Type: serum, plasma and other biological fluids
Assay Length: 3.5h
Research Area: Cardiovascular; Signal Transduction; Cardiovascular; Metabolism
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human hs-Hepc25. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human hs-Hepc25. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human hs-Hepc25, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human hs-Hepc25 in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (ng/mL) OD Corrected OD
100.00 1.821 1.725
50.00 1.438 1.342
25.00 1.065 0.969
12.50 0.900 0.804
6.25 0.461 0.365
3.13 0.376 0.280
1.56 0.218 0.122
0.00 0.096 0.000

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant hs-Hepc25 and the recovery rates were calculated by comparing the measured value to the expected amount of hs-Hepc25 in samples.
Matrix Recovery range Average
serum(n=5) 90-118% 104%
EDTA plasma(n=5) 88-105% 97%
Heparin plasma(n=5) 93-99% 96%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of hs-Hepc25 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 81-96% 83-118% 94-112% 92-111%
EDTA plasma(n=5) 86-97% 87-116% 82-98% 84-97%
Heparin plasma(n=5) 90-107% 93-106% 95-114% 93-99%

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