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Human hs-cTnI (High Sensitivity Cardiac Troponin I) Microsample ELISA Kit
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ELK9080MS
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48 T $412.00
96 T $588.00
96 T*5 $2499.00
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Overview

Product name: Human hs-cTnI (High Sensitivity Cardiac Troponin I) Microsample ELISA Kit
Reactivity: Human
Alternative Names: High Sensitivity Cardiac Troponin I
Assay Type: Sandwich
Sensitivity: 9.8 pg/mL
Standard: 2000 pg/mL
Detection Range: 31.25-2000 pg/mL
Sample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length: 3.5h
Research Area: Cardiovascular biology
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human hs-cTnI. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human hs-cTnI. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human hs-cTnI, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human hs-cTnI in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (pg/mL) OD Corrected OD
2000.00 2.270 2.182
1000.00 1.724 1.636
500.00 1.177 1.089
250.00 0.865 0.777
125.00 0.548 0.460
62.50 0.371 0.283
31.25 0.216 0.128
0.00 0.088 0.000

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant hs-cTnI and the recovery rates were calculated by comparing the measured value to the expected amount of hs-cTnI in samples.
Matrix Recovery range Average
serum(n=5) 91-111% 101%
EDTA plasma(n=5) 88-110% 99%
Heparin plasma(n=5) 94-104% 99%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of hs-cTnI and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 85-117% 87-94% 82-104% 81-102%
EDTA plasma(n=5) 87-110% 89-108% 81-119% 90-116%
Heparin plasma(n=5) 92-115% 90-100% 81-98% 90-118%

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