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Human hs-cTnT(High Sensitivity Cardiac Troponin T) Microsample ELISA Kit
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ELK8843MS
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48 T $320.00
96 T $458.00
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Overview

Product name: Human hs-cTnT(High Sensitivity Cardiac Troponin T) Microsample ELISA Kit
Reactivity: Human
Alternative Names: High Sensitivity Cardiac Troponin T
Assay Type: Sandwich
Sensitivity: 4.1 pg/mL
Standard: 1000 pg/mL
Detection Range: 15.63-1000 pg/mL
Sample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length: 3.5h
Research Area: Cardiovascular biology
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human hs-cTnT. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human hs-cTnT. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human hs-cTnT, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human hs-cTnT in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (pg/mL) OD Corrected OD
1000.00 2.036 1.956
500.00 1.521 1.441
250.00 1.009 0.929
125.00 0.879 0.799
62.50 0.556 0.476
31.25 0.350 0.270
15.63 0.130 0.050
0.00 0.080 0.000

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant hs-cTnT and the recovery rates were calculated by comparing the measured value to the expected amount of hs-cTnT in samples.
Matrix Recovery range Average
serum(n=5) 86-97% 92%
EDTA plasma(n=5) 82-108% 95%
Heparin plasma(n=5) 93-109% 101%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of hs-cTnT and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 93-106% 82-93% 82-107% 83-105%
EDTA plasma(n=5) 86-119% 92-97% 85-112% 89-96%
Heparin plasma(n=5) 81-118% 91-100% 91-113% 84-118%

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