Products

Mouse PGD2(Prostaglandin D2) ELISA Kit
ELK8759
Size： Price：
48 T $265.00
96 T $376.00
Manual MSDS Send email to us

Overview

Product name: Mouse PGD2(Prostaglandin D2) ELISA Kit
Reactivity: Mouse
Alternative Names: PG-D2
Assay Type: Competitive Inhibition
Sensitivity: 4.31 pg/mL
Standard: 1000 pg/mL
Detection Range: 15.63-1000 pg/mL
Sample Type: serum, plasma and other biological fluids
Assay Length: 2h
Research Area: Cell proliferation
Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Mouse PGD2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse PGD2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse PGD2 in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (pg/mL) OD Corrected OD
1000.00 0.235
500.00 0.396
250.00 0.715
125.00 0.823
62.50 1.236
31.25 1.572
15.63 1.769
0.00 2.197

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant PGD2 and the recovery rates were calculated by comparing the measured value to the expected amount of PGD2 in samples.
Matrix Recovery range Average
serum(n=5) 87-99% 93%
EDTA plasma(n=5) 92-105% 98%
Heparin plasma(n=5) 83-95% 101%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of PGD2 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 85-97% 93-101% 87-101% 89-102%
EDTA plasma(n=5) 93-105% 85-97% 90-99% 82-101%
Heparin plasma(n=5) 82-94% 92-104% 82-96% 95-102%