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Human sVEGFR-1(soluble Vascular Endothelial Growth Factor Receptor 1) ELISA Kit
ELK8719
Size： Price：
48 T $265.00
96 T $376.00
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Overview

Product name: Human sVEGFR-1(soluble Vascular Endothelial Growth Factor Receptor 1) ELISA Kit
Reactivity: Human
Alternative Names: Svegfr-1
Assay Type: Sandwich
Sensitivity: 3 pg/mL
Standard: 500 pg/mL
Detection Range: 7.82-500 pg/mL
Sample Type: serum, plasma, tissue homogenates, saliva, cell lysates, follicular fluid and other biological fluids
Assay Length: 3.5h
Research Area: Signal transduction;Tumor immunity;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human sVEGFR-1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human sVEGFR-1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human sVEGFR-1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human sVEGFR-1 in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (pg/mL) OD Corrected OD
500.00 2.054 1.952
250.00 1.527 1.425
125.00 1.034 0.932
62.50 0.835 0.733
31.25 0.527 0.425
15.63 0.352 0.250
7.82 0.267 0.165
0.00 0.102 0.000

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant sVEGFR-1 and the recovery rates were calculated by comparing the measured value to the expected amount of sVEGFR-1 in samples.
Matrix Recovery range Average
serum(n=5) 88-103% 95%
EDTA plasma(n=5) 95-107% 101%
Heparin plasma(n=5) 80-95% 87%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of sVEGFR-1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 95-104% 82-96% 92-105% 83-92%
EDTA plasma(n=5) 78-92% 83-96% 95-107% 87-102%
Heparin plasma(n=5) 82-94% 85-96% 78-90% 91-99%