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Human sTNF-R1(soluble tumor necrosis factor receptor 1) ELISA Kit
ELK8639
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48 T $412.00
96 T $588.00
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Overview

Product name: Human sTNF-R1(soluble tumor necrosis factor receptor 1) ELISA Kit
Reactivity: Human
Alternative Names: sTNF-R1; soluble tumor necrosis factor receptor 1
Assay Type: Sandwich
Sensitivity: 3.9 pg/mL
Standard: 1000 pg/mL
Detection Range: 15.63-1000 pg/mL
Sample Type: serum, plasma and other biological fluids
Assay Length: 3.5h
Research Area: Cancer
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human sTNF-R1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human sTNF-R1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human sTNF-R1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human sTNF-R1 in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (pg/mL) OD Corrected OD
1000.00 1.916 1.837
500.00 1.643 1.564
250.00 1.107 1.028
125.00 0.852 0.773
62.50 0.516 0.437
31.25 0.381 0.302
15.63 0.237 0.158
0.00 0.079 0.000

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant sTNF-R1 and the recovery rates were calculated by comparing the measured value to the expected amount of sTNF-R1 in samples.
Matrix Recovery range Average
serum(n=5) 90-105% 97%
EDTA plasma(n=5) 97-105% 101%
Heparin plasma(n=5) 95-107% 101%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of sTNF-R1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 79-91% 87-96% 83-101% 82-95%
EDTA plasma(n=5) 85-93% 87-96% 97-105% 95-102%
Heparin plasma(n=5) 83-92% 96-102% 81-93% 79-91%